- Edward Maria Wingfield (1607) one of four incorporators of the London Virginia Company, who financially helped fund the colony;
- John Ratcliffe (1608), Captain of the Discovery, who was killed by Indians;
- Matthew Scrivener (1608) who drowned in a storm crossing over to Hog Island;
- John Smith (1608–1609) fought the Muslim Ottoman Empire in Eastern Europe before settling Jamestown; he was almost killed by Indian Chief Powhatan but rescued by the Chief's daughter, Pocahontas;
- George Percy (1609–1610, March–May 1611) helped the Dutch fight for independence from Spain; he was Jamestown's governor during the "Starving Time";
- Sir Thomas Gates (May–June 1610, 1611–1613) knighted for his gallantry in the capture of Cadiz, Spain; was shipwrecked with the Sea Venture on the Island of Bermuda for 10 months, the written account of which inspired Shakespeare's play, The Tempest.
- Thomas West, Baron De La Warr (1609–1618) member of the King's Privy Council, arrived in Jamestown just in time to save the colony from abandonment; namesake of the Delaware River and Colony;
- Sir Samuel Argall (1617–1619) a sea captain, knighted by King James for hindering French colonization of Acadia, Canada, and Algiers, North Africa; noted for kidnapping Pocahontas who John Rolfe fell in love with and married, restoring relations with the Powhatan Tribe;
- Sir Thomas Dale (May–August 1611, 1613–1616) naval commander who helped Dutch fight for independence from Spain, knighted by King James; was sent by the London Company to Virginia in May of 1611. He cancelled Virginia Company instructions of "communal property" and assigned each colonist their own plot of land; he sailed with John Rolfe and his wife Pocahontasback to England.
Dale sailed up the James River and founded Henricus, the colony’s second settlement, also named after James' eldest son, Prince Henry.
In 1619, Henricus became the location of the first English hospital in America and the first chartered college in the English colonies, initially designed for Powhatan children to learn trades, agriculture, and Christianity, "the work of conversion."
Unfortunately, it was destroyed in the 1622 Indian uprising.
- Sir George Yeardley (1616–1617, 1619–1621) shipwrecked with the crew of the Sea Venture on Bermuda; he convened a meeting in Jamestown's church choir which is considered the first representative legislative assembly in the New World, and became the Virginia House of Burgesses.
- Sir Francis Wyatt (1621–1624) knighted by King James; helped defend Jamestown from 1622 Indian attack which killed 347 settlers; he wrote a constitution specifying colonists' privileges, and helped found Maryland.